Scientists Confirm Two Domesticated Buffalo Species in PH

Scientists Confirm Two Domesticated Buffalo Species in PH

Published: September 17, 2024

By: Eunice Jean C. Patron

Domesticated buffaloes (Bubalus bubalis) in the country have traditionally been classified into two subspecies: swamp buffaloes and riverine buffaloes. A recent study, however, provides evidence that these two are not subspecies, but are instead distinct from each other.

A Philippine native carabao (Bubalus kerabau Fitzinger, 1860) dam and her calf grazing in Pitogo Island, Bohol.

(Photo credit: Lilian P. Villamor, from Ruane et al., 2023)

Swamp buffaloes (Bubalus bubalis carabanensis) are primarily used to assist farmers with tilling the land, transporting tools, and, alternatively, for meat, milk, hide, and manure, which is used as organic fertilizer. Riverine buffaloes (Bubalus bubalis bubalis), on the other hand, are mainly used in milk production. In the Philippines, crossbreeding and backcrossing these two breeds on a large scale helps create animals with improved potential for milk and meat production, despite their differences. Hybridization of swamp and river buffaloes has limited success, sometimes producing viable offspring that don’t achieve the desired traits.

 

A team of researchers conducted an investigation to clarify the taxonomic designation of swamp and riverine buffaloes in the Philippines. They discovered that the DNA sequences of the genes from both swamp and riverine buffaloes are distinguishable and that their chromosome numbers are different, affirming that they are two distinct species, not just subspecies of Bubalus bubalis.

 

The research team is comprised of Dr. Ian Kendrich C. Fontanilla, Nelvie Fatima Jane A. Soliven, John Gregor A. Roño, Francis L. Fontanilla, Emerson R. Servo, and Dr. Ernelea P. Cao of the University of the Philippines – Diliman College of Science’s Institute of Biology (UPD-CS IB) and Dr. Lilian P. Villamor, Therese Patricka C. Cailipan, Alexander M. Paraguas, and Aivhie Jhoy E. Cuanang from the Department of Agriculture – Philippine Carabao Center (DA-PCC).

 

“These results corroborate the observed differences in their appearances. Swamp buffaloes can be identified by the white or light gray markings on their lower jaw and brisket, known as a chevron, as well as their lighter skin and coat color compared to riverine buffaloes. They are also smaller, weighing between 325 and 450 kg, and have crescent-shaped horns. They are mainly used as draught animals,” said Dr. Villamor, citing data from her previous studies on buffaloes. “River buffaloes, on the other hand, are larger, weighing between 450 and 1,000 kg, and have curly horns. They are primarily raised for milk and meat production.”

A riverine buffalo (Bubalus bubalis Linnaeus, 1758). (Photo credit: Therese Patricka C. Cailipan and Sonny C. Ramos)

According to the team, the Philippine native carabao, which is currently classified as Bubalus bubalis carabanensis, should be now considered as Bubalus kerabau Fitzinger, 1860. “The reclassification of the Bubalus bubalis carabanensis to Bubalus kerabau Fitzinger, 1860 supports earlier assumptions that riverine and swamp buffaloes are distinct,” added Dr. Fontanilla. “Confusions on the classifications of the two buffaloes have implications on the breeding program of these species.”

 

As their study was limited to examining the chromosomes of only swamp and riverine buffaloes, the researchers plan to include chromosomes from crossbred buffaloes in future studies to gain new insights into how these different types of buffaloes are genetically related and potentially improve the classification of the Bubalus bubalis.

 

The study, titled “Molecular Data and Karyotype Revealed Two Distinct Species of Domesticated Water Buffaloes in the Philippines,” offers valuable insights into the diversity of domesticated buffaloes, and can guide conservation and management efforts, particularly in developing potential hybrids with desired traits. It was published in the Philippine Journal of Science, an open-access, single-blind peer-reviewed journal on natural sciences, engineering, mathematics, and social sciences.

 

For interview requests and other concerns, please contact media@science.upd.edu.ph.

 

References:

 

Cailipan, T. P., Paraguas, A., Cuanang, A. J., Soliven, N. F., Roño, J. G., Fontanilla, F., Servo, E., Cao, E., Fontanilla, I. K., & Villamor, L. (2023). Molecular data and karyotype revealed two distinct species of domesticated water buffaloes in the Philippines. Philippine Journal of Science, 152(5). https://doi.org/10.56899/152.05.27

 

Cailipan, T.P.C., Villamor, L.P. 2021.  Molecular characterization of SRY gene in the Philippine Carabao (Bubalus bubalis) swamp buffalo populations. SciEnggJ 14(2):291-296.

 

Ruane, J., Mba, C., Boettcher, P., Koskela, J., Mair, G., & Ramasamy, S. (2023). Case studies of the use of agricultural biotechnologies to meet the needs of smallholders in developing countries. Food & Agriculture Org.

 

Villamor, L.P., Takahashi, Y., Nomura, K. and Amano T. 2021. Genetic diversity of Philippine Carabao (Bubalus bubalis) using mitochondrial DNA D-loop variation: implications to conservation and management. Philippine Journal of Science, 150(3): 837-846.

Here’s How UP Physicists are Helping with the Hunt for Gravitational Waves

Here’s How UP Physicists are Helping with the Hunt for Gravitational Waves

Published: September 13, 2024

By: Harvey L. Sapigao

A black hole inside an accretion disk. (Photo credit: BoliviaInteligente on Unsplash)

In 1916, Albert Einstein theorized that two merging black holes create ripples in the spacetime fabric, similar to how a pebble creates ripples in a pond. These ripples, called gravitational waves, stretch and squeeze spacetime in amounts so minuscule that they were once believed to be too faint to detect.

 

But a century later, the Laser Interferometer Gravitational-Wave Observatory (LIGO) in the US, an L-shaped facility with arms spanning four kilometers each, detected minute discrepancies in how long lasers travel through each arm, signaling the first detection of gravitational waves.

 

Now, scientists are preparing to launch a more sophisticated observatory into space, aiming to detect even fainter gravitational waves or those beyond LIGO’s capabilities. This space-based facility, known as the Laser Interferometer Space Antenna or LISA, is a triangular observatory with sides spanning tens of millions of kilometers and is set to launch in the 2030s.

 

As preparation ramps up, scientists around the world are pitching ideas to improve LISA’s detection capabilities. Dr. Reinabelle Reyes and her former graduate student Marco Immanuel Rivera, from the UP Diliman College of Science’s National Institute of Physics (UPD-CS NIP) recently published a study identifying a set of parameters that could improve the analysis of signals coming from LISA and future gravitational-wave observatories.

 

Unlike LIGO, which mainly detects gravitational waves coming from two stellar-mass black holes, LISA hopes to detect a type of gravitational wave coming from compact objects – such as neutron stars, white dwarfs, and stellar-mass black holes – orbiting supermassive black holes. “When a stellar-mass black hole orbiting a supermassive black hole falls into it, an extreme-mass ratio inspiral (EMRI) gravitational-wave signal is produced,” Dr. Reyes and Rivera explained.

 

One complication with detecting EMRIs is that the environment where the compact object-black hole pair resides can considerably affect the EMRIs they emit. For example, supermassive black holes are often surrounded by glowing rings called accretion disks, which can modify the EMRI signal just as the Earth’s atmosphere distorts light from faraway stars.

 

By understanding how these environmental features affect EMRIs, astronomers can not only filter out noise from the signal but also learn about the environment itself. For instance, by studying the imprints of the environment on the gravitational wave signal, astronomers can infer the density of the accretion disk. 

 

Their study considered three environmental factors that may substantially influence the EMRI signal: accretion, gravitational drag, and gravitational pull. Their analysis determined the most measurable parameter combination, which is heavily dominated by these environmental effects. They also estimated how precisely these parameters can be measured—an essential factor for extremely sensitive detectors like LISA.

 

Their analysis is built upon a mathematical tool called the Fisher matrix, which evaluates how accurately an experiment can measure different observables. To illustrate, imagine a catch basin designed to collect water, rocks, and leaves. The Fisher matrix determines and quantifies how effectively the basin can catch each object separately, even before the experiment is set out.

 

“The Fisher matrix is used by astrophysicists to estimate the expected precision to which certain properties can be measured from a given signal to be observed in a future detector,” explained the authors.

 

While their study shows promise, Dr. Reyes and Rivera noted that modeling EMRIs is challenging due to strong gravity effects, and more accurate modeling is needed.  “It will be interesting to compare with calculations based on the newer waveform models which are adapted for EMRIs, as well as those which contain the effects of non-trivial environments,” the authors said.

 

“In the future, we hope to see how the parameter combinations we presented in this study can be applied directly in improving parameter estimation methods used in gravitational-wave astronomy, such as stochastic samplers,” the authors concluded.

 

For interview requests and other concerns, please contact media@science.upd.edu.ph.

 

Rivera, M.I. and Reyes, R.C. (2024) Measurable parameter combinations of environmentally-dephased EMRI gravitational-wave signals, New Astronomy, 112, p. 102263. https://doi.org/10.1016/j.newast.2024.102263

UP Researchers Investigate E. coli Levels in Urban Farm Vegetables

UP Researchers Investigate E. coli Levels in Urban Farm Vegetables

Published: August 27, 2024

By: Eunice Jean C. Patron

A type of bacterium called Escherichia coli (E. coli), found in the feces of humans and animals, is often released into bodies of water such as streams. Although most E. coli strains are harmless, some can cause foodborne diseases such as gastroenteritis, typhoid fever, and dysentery. Feces are a major source of microbial contamination in fresh produce. With the rise of urban farming in the Philippines during the COVID-19 pandemic, it is essential to monitor fresh produce from urban farms to recognize and address food safety issues immediately.


Scientists from the University of the Philippines – Diliman College of Science (UPD-CS), supported by the Department of Agriculture – Bureau of Agricultural Research (DA-BAR), assessed vegetables from wet markets and urban farms in Metro Manila during the peak of the pandemic to detect and quantify the presence of E. coli.

Researchers gather samples of mustasa, or mustard greens (Brassica juncea), at New Greenland Farm in Bagong Silangan, Quezon City. (Photo credit: The Research Team)

Dr. Pierangeli Vital, Donnabel Sena, Czarina Jay Catapat, and Ma. Christine Jasmine Sabio from the UPD-CS Natural Sciences Research Institute (UPD-CS NSRI), along with Dr. Windell Rivera of the UPD-CS Institute of Biology (UPD-CS IB) gathered 419 vegetable samples from three urban farms and four major wet markets in Metro Manila. The group detected E. coli in 13.60% of all the samples. The presence of E.coli in the samples from urban farms was also higher than in the samples obtained from wet markets.

 

According to Dr. Vital, high levels of E. coli in the samples from urban farms could be linked to various factors, such as lack of thorough post-harvest processing, unlike the samples from wet markets. “As observed in the urban farm sampling sites, they frequently use rainwater, pond water, and well water for irrigation, mainly for economic reasons, but this increases the risk of bacterial contamination,” she said.

 

Animals such as dogs, cats, and chickens often roam around urban farms, which raises the risk of microbial contamination in the irrigation water and soil where the vegetables grow. The researchers considered these as areas for improvement in developing urban farming practices.

 

“This research, together with our past research on agricultural food safety, is and was used in creating and adopting Philippine National Standards (PNS) on pre- and post-harvest microbial food safety in agriculture, with initiatives spearheaded by the DA – Bureau of Agriculture and Fisheries Standards (DA-BAFS),” shared Dr. Vital.

 

The researchers stated in their paper that the results could provide a factual basis for regulating, controlling, and eliminating food pathogen contamination in fresh produce, thereby helping policymakers implement regulations that ensure food safety. Even so, Dr. Vital mentioned that preventing foodborne illnesses such as E. coli can also be done by Filipinos in their own homes.

 

“Proper handling and cooking must be practiced at home, which involves simple steps such as thoroughly washing hands and kitchenware as well as disinfecting table surfaces when preparing food, using clean water when washing vegetables, effectively separating cooked and raw foods, and storing food the right way,” Dr. Vital added. “Following these simple steps can significantly help reduce the risks of foodborne illness at home and in the community.”

 

They plan to include more surveillance of relevant foodborne pathogens and conduct the study on a national level, in addition to investigating the antimicrobial resistance of the bacteria. These endeavors will provide significant data for analyzing trends in microbial food safety in the Philippines.

 

The paper, titled “Thermotolerant Escherichia coli contamination in vegetables from selected urban farms and wet markets in metro Manila, Philippines at the height of COVID-19 pandemic,” was published in the Asia-Pacific Journal of Science and Technology, a peer-reviewed journal covering research fields such as engineering, agricultural sciences, technology, and health sciences.

 

For interview requests and other concerns, please contact media@science.upd.edu.ph.

 

References:


Vital, P., Rivera, W., Sena, D., Catapat, C. J., & Sabio, M. C. J. (2024). Thermotolerant Escherichia coli contamination in vegetables from selected urban farms and wet markets in Metro Manila, Philippines at the height of COVID-19 pandemic. Asia-Pacific Journal of Science and Technology, 29(03), APST–29. https://doi.org/10.14456/apst.2024.44

Response of behalf of the MS/PhD Graduates

Response on behalf of the MS/PhD Graduates

To our guest speaker, National Artist Kidlat Tahimik, to our Chancellor, Chancellor Vistan, to our dean, Dr. Giovanni Tapang, academicians, university officials, institute directors, faculty, parents, guests and of course graduates, magandang gabi po sa ating lahat.


The theme for today’s commencement is “Science in the Service of Society.” Since I’m not particularly experienced in writing speeches, I’ll approach this topic the way we tackle complex scientific journals—by defining and discussing each term.


Let’s start with the word “science.” After spending at least four years immersed in this field, I believe its meaning is already familiar to us. However, I’d like to share a glimpse of the state of science in our country based on my experiences.


My adviser, Dr. Nathaniel Hermosa came back to the Philippines from his postdoctoral position as a Balik-Scientist fellow to establish his own laboratory in 2015. I was fortunate to be one of his first advisees. I witnessed firsthand the process of building a laboratory from the ground up, including the relatively tedious process of procuring equipment. I have seen how researchers, including my mentors, often have to justify their work as addressing pressing issues like poverty just to secure financial support in their projects. I, myself have experienced being disheartened with these circumstances. Last 2023, our work was selected as a finalist for the Best Student Research Award in Spain. Despite the university’s generous support, I was unable to attend the conference in person due to inadequate funding.


Let me be clear: our university is incredibly supportive and generous with research funding, but these challenges are part of the reality we face. Minsan nakaka-frustrate, madalas nakaka-lungkot. Yet, despite these obstacles, we continue to excel, producing great research papers and nurturing world-class scientists.


During one research meeting, I asked my adviser, “Why did you choose to return and conduct experiments here in the Philippines when it might be easier to do so abroad?” His response, in essence, was that while we might lack in resources and funding, we make up with ingenuity and resourcefulness. This may be an adaptive skill, but our lack forces us to think outside the box and come up with creative solutions to problems.


Next word is service. For this part, let me tell you a story.


Tungkol ito sa isang mag-asawang magsasaka na nagngangalang Ceasar at Aurora. Sila ay may tatlong anak: isang panganay na babae at dalawang lalaki. Upang mapagtapos ang kanilang mga anak, kinailangan nilang maghanap ng iba pang trabaho bukod sa pagsasaka. Nagsimulang mag-buy and sell si Ceasar ng mga baboy, at naging manininda naman sa palengke si Aurora. Ang kanilang panganay na anak na si Mona ang katulong ng kanyang ina sa pagtitinda sa palengke.


Sa murang edad, namulat si Mona sa hirap ng buhay at sa sakripisyo ng kanyang mga magulang upang mapag-aral silang magkakapatid. Sa buong apat na taon ng high school, mayroon lamang siyang isang pares ng uniporme at isang pares ng sapatos na pinilit niyang pinagkakasya sa kanyang lumalaking paa. Nakapagtapos ng high school si Mona, ngunit dahil sa hirap ng buhay, sinabihan siya ng kanyang ina na baka hindi na nya ito kayang suportahan sa pag-aaral nito sa kolehiyo. Pursigidong makatapos ng pag-aaral si Mona, kaya naghanap siya ng paraan upang tulungan ang kanyang sariling makapag tapos. Nag-simula syang magbenta sa kanyang mga classmates ng kanyang mga paninda. Nag tiis syang mag-meryenda sa isang pirasong muncher o tattoos habang pinapanood ang kanyang mga kaklaseng kumain ng softdrinks at piattos. Sa awa ng Diyos, nakakuha sya ng scholarship at tuluyang nakapagtapos ng kolehiyo.


This story is not mine but that of my mother’s and grandparents’. My mother was the first in her clan to earn a college degree. I am the first in our immediate family to graduate in UP.


We should remember that these diplomas and degrees are not solely the result of our individual efforts. We are here because of the collective support and sacrifices of those who stood by us throughout this journey. We are reaping the fruits of their labor and dedication.


Where does service fit into all this? Service and sacrifice go hand-in-hand. True service is never comfortable; it involves sacrificing something of our own and going out of our way to help others.


My grandfather Ceasar, who passed away during the 2020 pandemic, is not here to witness this moment. While we may never fully repay the debt we owe to those who came before us, we can certainly pay it forward. We can become mentors to the next generation of scientists. We can serve our community with our knowledge and skills. We can conduct research that advances science for the betterment of society.


Finally, let’s reflect on the word “society.”


I had a hard time concentrating on writing this speech due to the recent flood that has affected so many this week. In the middle of the typhoon last Wednesday, my husband had to rescue our pamangkins in Cainta because the flood is already approaching their second floor. It is really challenging to celebrate today however, I believe this is precisely the moment to reflect on the true meaning of this ceremony.


I took some time to research the meaning behind our UP Sablay. According to OUR’s website, the word “Sablay” refers to an indigenous loose garment that, though simple, is traditionally worn for formal occasions. As a verb (isablay), it means to drape a precious object, such as this garment, over one’s shoulder, symbolizing value and respect for that object.


Today, we are giving value and honoring not only our efforts but also the people who have paved the way for us—our seniors, mentors, and the entire community who helped us wear this Sablay with pride. We are showing respect for the dedication and service of everyone who made our journey possible.


Our UP Sablay represents more than personal achievement; it symbolizes our responsibility to contribute to society. As we wear our Sablays today, let us remember that our journey is a testament to the collective effort and dedication of our community. It’s a call to use our knowledge, skills, and achievements to serve others, to uplift those around us, and to address the pressing needs of our society.


As my call to action, I want to repeat what I said in our institute’s recognition this morning.

Pagsilbihan ang kapwa, pagsilbihan ang sambayanan. Gamitin natin ang pananaliksik bilang instrumento ng pagbabago.


Let’s use our education in paying it forward and may our legacy be one of bettering the next generation.


Thank you, and congratulations to the College of Science Class of 2024. Mabuhay ang mga siyentista ng bayan.

Response of behalf of the BS Graduates

Response on behalf of the BS Graduates

Maganda’t mabuting araw sa inyong lahat! Ako si Sean Brendan, mula sa Pambansang Linangan ng Pisika.


Tuwing iniisip ko kung bakit ba ‘ko nandito sa pisika’t agham, palagi akong walang depinitibong sagot. No’ng bata ako pinangarap ko maging astronot. ‘Di ko alam. Baka dahil ang astig ng kanilang damit, tapos sasakay sa ispeyship. Ang lupit din tingnan ng mga kemistri-kemistri. Pati ‘yang mga daynosor na ‘yan. Palaging paiba-iba ang gusto ko.

Bilang mga siyentista ng hinaharap, na minsa’y sinilaw rin ng mga bitwin, numero, palaka, o kung anumang dahilan kung bakit tinahak ang daang siyensya, kahit sa’n pa man tayo dalhin ng ating pagtuklas, alam nating madaling malunod sa angking ganda nito—sa sarap ng pakiramdam tuwing nakaka-solb tayo ng ekweysyon, sa pagpaypet ng kemikal, sa pagdila ng bato, o maging sa pakiramdam na, …, basta, “ang kul pakinggan”. Pero minsan, malungkot. Totoo ang mga nararamdaman nating kalungkutan sa “‘di tayo napili”, “‘di tayo nanalo”, “‘di tayo nakapasa kasi mababa ang gan’to”. “Kapag ba nagpatuloy ako sa
siyensya, magiging matagumpay pa ba ‘ko kung ngayon palang, ‘di na ‘ko magaling?”

Kadalasang ikinakabit ng mga pamantayang internasyual ang tagumpay sa agham sa ating h-index, o kung anumang iskolarling batayan sa bilang ng ating mga nailathala, kung ilang beses ito isinipi, at iba pa. Ang iba nama’y ikinakabit ang tagumpay sa kung ga’no kataas ang ating makakamit, sa rekognisyon at kasikatan na makukuha natin sa akademikong komunidad mula sa pagtuklas ng mga bagong resulta o pagbuo ng mga abstraktong teorya. Ang iba pa’y ikinakabit ang tagumpay sa kung ga’no kalayo ang ating mararating, kung ga’no kalaki’t maimpluwensiya ang mga prestihiyosong institusyong akademiko o industriyal ang ating mapupuntahan. Maraming pagsasalarawan sa iba’t ibang puntong pibotal na sumusukat sa kung ga’no tayo katagumpay sa ating mga larangan. Kung ngayon sa mga linangan, mga grado, sa akademya’t industriya, marami pang metrikong ginagamit para sukatin tayo. Bagamat nagagamit ito sa pagsukat ng kasanayan, pananagutan, pagpuna, pagdedesisyon, at pagpapabuti, ang mga ito rin ay nagiging kawnterproduktib.

Sa ganitong kapaligiran, ang presyur na umayon ay maaaring nakapaghihina sa pagkamalikhain. Ang mismong diwa ng siyentipikong pagdiskubre—ang pagsasaliksik at paghahamon sa istatus kwo—ay napahihina ng walang tigil na paghahabol sa mga paborableng sukatan. Gayunpaman, maging sa harap nitong mga mahihirap at ‘di magagandang realidad sa mundo ng agham na nagdudulot ng malawakang kumpetisyon sa trabaho at paghina ng produktibong gawa—sa mukha ng krisis sa edukasyon, limitadong akses sa impormasyon, kakulangan sa pondo, at sa kadiliman ng klimang pulitikal—hindi pa rin dapat na kalimutan ang mga mamamayan.


Habang nauunawaan natin na ang mga pananaliksik ay may kanya-kanyang kalakasan, sakop, at limitasyon—may direktang impak man sa mga komunidad at napapanahong isyu, o wala dahil ito’y abstrak at wala pa masyadong nakaiintindi—nais kong ipaalala sa lahat na ang ating pananaliksik, ang kabuuan ng ating trabaho, ay hindi umiiral isang bakyum. Ito’y isang intigral na bahagi ng mas malawak pang ekosistemang kinabibilangan din ng mga taong sumusuporta sa ating pananaliksik at ng publiko na ang buhay ay nais nating pagbutihin no’ng una pa lamang. Kaya kahit ano pa man ang sabihin ng mga
sukatan tungkol sa kakayahan at tagumpay natin, h’wag nating hayaan na magtabon ito sa tunay na pagkamalikhain, sa tunay na paghahangad ng kaalaman, at syempre sa integridad ng agham, dahil ang bayan ay nakasandal sa atin.


At habang naiintindihan din naman natin na ‘di makapagbubuhos ang walang laman na bukal—na tayo ri’y napapagod, nauubos, nalulubog sa kahirapa’t, nawawala ang pangarap para sa mamamayan dahil sa minsang pangangailangang unahin ang sarili—nais ko ring ipaalala na ang ating pagpupunyagi ay magkakaroon pa rin ng mas malalim na kahulugan. Kapag sumikat na muli sa wakas ang araw sa napakatagal at napakadilim nating gabi, makababalik din tayo sa pagpapanday ng landas para sa mga susunod na henerasyon.
At ‘di rin dapat natatapos ang siyensya sa papel at pagsasaayos ng mga metriko. ‘Di rin sa pagbuo ng mga aplikasyon nito.


H’wag tayong magtapos sa pagtutok lamang sa ating mga pag-uusap sa isa’t isa rito, sa pagbabahagi sa mga kapwa lamang na siyentista sa mga kumperensya’t kumbensyon, kundi iparating din natin ang ating mga natutuklasan sa mga lente ng mga nararanasang realidad na nakaaantig sa mga tao. Hindi sapat na alam lamang natin na ang ating ginagawa ay para sa tao, para sa bayan, naiuugnay din dapat natin ito sa adbokasiya’t aktibismo. Dapat naisasalin din natin ang mga kumpleksiting ito sa mga malilinaw at makabuluhang naratibong akesibol para sa mga bata, sa mga matatanda, sa mga taong nasa ibang larangan, sa mga taong may limitadong kakayahan, maging sa mga taong kinaaayawan—para sa lahat—dahil ang ating agham ay ang ating pagmamahal, at ang ating pagmamahal ay mapagbuklod at mapagpalaya.


Iparinig at iparamdam natin ang ating agham.

UP Scientists Explore Non-Gaussian Laser Effects

UP Scientists Explore Non-Gaussian Laser Effects on Metal Heat Diffusion

Published: July 30, 2024

By: Eunice Jean C. Patron

While existing research on material properties rarely examines changes in laser pulses, or the flashes of laser lights, over time, scientists from the University of the Philippines – Diliman College of Science (UPD-CS) approached laser exploration differently by investigating how non-Gaussian lasers affect the diffusion of heat in metals.

Pulsed laser ablation of a material inside a vacuum system. (Photo credit: Dr. Lean Dasallas)

In their recent study, Jenny Lou Sagisi and Dr. Lean Dasallas of the UPD-CS Materials Science and Engineering Program (UPD-CS MSEP), along with Dr. Wilson Garcia of the UPD-CS National Institute of Physics (UPD-CS NIP), investigated the effects of different temporal laser source terms (TLSTs), or shapes of the laser pulses, on simulating heat diffusion in metals.

 

Validating Gaussian Assumptions in Studies

 

Dr. Dasallas explained that scientists often assume laser pulses to light up in a bell-shaped curve (known as Gaussian): “Dim at first, but slowly brightening until it becomes super bright in a very short time, before dimming again,” he further expounded. This assumption is common and accepted in laser research due to the laser pulse’s duration, which is one quadrillionth (10-15) of a second.

 

Simulations conducted by the scientists to compare the differences between Gaussian-shaped and non-Gaussian-shaped laser pulses showed that the rough edges on a non-Gaussian laser pulse make it difficult for the laser to vaporize the metal. They also noted that consecutively using laser pulses shows effects on metals that are not as distinct as those seen with Gaussian-shaped laser pulses.

Comparison of Gaussian and non-Gaussian laser pulses in intensity over time. (Photo credit: Sagisi et al., 2024)

“We were curious to see if there are any changes in the laser pulse’s effect on heat diffusion in metals when they are not Gaussian-shaped,” Dr. Dasallas shared. “Through our computer simulations, we were able to validate that assuming a Gaussian-shaped laser pulse has a basis. It also turns out that the shape of the laser pulses won’t matter if there are many of them.”

 

Implications in Industry

 

According to the researchers, exploring the effects of non-Gaussian laser pulses is vital for optimizing laser processing methods in more precise applications such as micro- or nano-machining, surface modification, and nanoparticle generation. Becoming more acquainted with non-Gaussian-based lasers also allows for more options and tools in laser material processing.

 

Extending this work to other materials, such as semiconductors, polymers, and insulators, would be very interesting. We plan to continue improving our understanding of complex laser-material interactions, which could involve experimental, theoretical, or computational approaches,” Dr. Dasallas said as he shared their team’s goals for research on laser-based material processing. “The laser industry is now worth billions of dollars. Various technologies already employ lasers, and the Philippines is catching up with these technologies.”

For interview requests and other concerns, please contact media@science.upd.edu.ph.

 

References:

Sagisi, J. L., Garcia, W. O., & Dasallas, L. L. (2024). Modification of the temporal laser source term in two-temperature model. Numerical Heat Transfer, Part A: Applications, 1-18. https://doi.org/10.1080/10407782.2024.2350687

UP Study Pinpoints Key Player in Aggressive Type of Prostate Cancer

UP Study Pinpoints Key Player in Aggressive Type of Prostate Cancer

Published: July 19, 2024

By: Harvey L. Sapigao

Schematic diagram of protein CYB561 (oblong figure in center) in action. CYB561 processes some iron into its more active form, contributing to the active iron pool (Photo credit: Azur et. al. 2024).

According to the World Health Organization, prostate cancer is the third most common type of cancer among Filipino men. In 2022, almost 10,000 Filipino men were diagnosed with the disease. In serious cases, the disease can develop into an aggressive type called castration-resistant prostate cancer (CRPC). Some further progress into the most aggressive form, neuroendocrine prostate cancer (NEPC), where standard treatments no longer work and it becomes the most fatal of all prostate cancers.

 

In a pioneering study, Romie Angelo Azur, Kevin Christian Olarte, Weand Ybañez, Alessandria Maeve Ocampo, and Dr. Pia Bagamasbad of the UP Diliman College of Science National Institute of Molecular Biology and Biotechnology (UPD-CS NIMBB) identified and described a key protein that contributes to the progression of prostate cancer into NEPC, elucidating the molecular basis of the disease and paving the way for identifying novel treatments.

 

Prostate cancer depends on male sex hormones, or androgens, to grow. When the prostate cancer cells don’t have access to androgens, their development is stunted. This is why androgen deprivation therapy (ADT) is the standard treatment for the disease.

 

Over time, however, the cancer cells can mutate in ways that enable them to develop without the need for androgens. This advanced type is resistant to ADT and may develop into the most aggressive form, the NEPC. Dr. Bagamasbad and her team discovered that a protein called CYB561 is pivotal for the progression, growth, and survival of aggressive, treatment-resistant prostate cancer cells.

 

“CYB561 has a dual role in driving cancer,” said Dr. Bagamasbad. “It activates specific growth factors and manages iron levels, both of which appear to help the cancer thrive and grow even when deprived of the male hormones it usually depends on.”

 

Using publicly available data from prostate tumors and experimental findings from human cell culture lines, they discovered that CYB561 is more prevalent in CRPC and NEPC cells than in normal prostate cancer cells. When they depleted CYB561, the prostate cancer cells became more sensitive to enzalutamide, a common ADT drug, suggesting that the protein provides some resistance to the drug.

 

Furthermore, they found that CYB561 converts iron into a more active form essential for various cell processes, including supporting the growth of aggressive forms of cancer. As such, the cancer cells require higher iron levels, and CYB561 seems to help maintain the needed active iron concentration. Knocking down the protein lowered active iron levels in NEPC cells, delayed the progression of CRPC to NEPC,  and dampened the highly aggressive behavior of NEPC cells.

 

The findings of their study, now published in PLOS One, can help develop new therapies for CRPC and NEPC. “By understanding the role of CYB561 in prostate cancer,” said Dr. Bagamasbad, “we have not only gained a deeper understanding of how prostate cancer develops drug resistance but we have also potentially identified a new target for future treatments, paving the way for novel therapies that could specifically inhibit CYB561’s activity to slow down or stop cancer progression.”

 

In the future, Dr. Bagamasbad and her team hope to experiment on animal models and primary tumor samples. They also plan to examine whether Filipinos have higher risks of developing CRPC and NEPC, and if CYB561 contributes to the aggressiveness of the disease. “More importantly, we need to identify other key players involved and establish a drug screening platform that can mechanistically target CYB561 activity,” Dr. Bagamasbad concluded.

 

For interview requests and other concerns, please contact media@science.upd.edu.ph.

 

References:

 

Azur RAG, Olarte KCV, Ybañez WS, Ocampo AMM, Bagamasbad PD (2024) CYB561 supports the neuroendocrine phenotype in castration-resistant prostate cancer. PLOS ONE 19(5): e0300413. https://doi.org/10.1371/journal.pone.0300413

Ferlay J, Ervik M, Lam F, Laversanne M, Colombet M, Mery L, Piñeros M, Znaor A, Soerjomataram I, Bray F (2024). Global Cancer Observatory: Cancer Today. Lyon, France: International Agency for Research on Cancer. Available from: https://gco.iarc.who.int/today, accessed 15 July 2024.

Latest PH Marine Mammal Stranding Hotspots and Species Stranding Status Entail Urgent LGU Response

Latest PH Marine Mammal Stranding Hotspots and Species Stranding Status Entail Urgent LGU Response

Published: July 15, 2024

By: Eunice Jean C. Patron

With a significant number of marine mammals stranded in the Philippines over the past years, it is within the scope of local government units (LGUs) to address this pressing concern, scientists from the University of the Philippines – Diliman College of Science (UPD-CS) indicated in a recent study that encompasses data from the past almost two decades.

A rare ginkgo-toothed beaked whale (Mesoplodon ginkgodens) was stranded alive in Subic Bay on April 19, 2010. Annual stranding frequencies in the Philippines generally increased from 29 in 2005-2007 to 106 in 2020-2022. (Photo credit: PMMSN)

Dr. Lemnuel Aragones, Alessandra Nicole Morado, and Honey Leen Laggui of the Institute of Environmental Science & Meteorology (IESM), along with Dr. Marie Christine Obusan of the Institute of Biology (IB), Dr. Jonah Bondoc of the Natural Sciences Research Institute (NSRI), and Dr. Leo Suarez of Ocean Adventure and Ewen Lawler of University of Canberra, assessed the changes in marine mammal strandings in the Philippines over space and time, as well as the types of species that got stranded in Philippine territory from 2005 to 2022.

 

Marine mammal strandings and the role of LGUs

 

A marine mammal stranding occurs when a marine mammal is found on seashores or in shallow waters and cannot return to deeper waters on its own. The researchers mapped the stranding hotspots in the Philippines, representing areas where standing frequencies are relatively high, and designed the hotspots to identify designated LGUs to inform them about the implications of the strandings for marine mammal conservation and management. With the help of the hotspots, concerned LGUs can also be proactive in addressing marine mammal strandings. A total of 35 LGU hotspots for strandings were identified in this study. The majority of these stranding hotspots were located in Luzon (with 24 hotspots), particularly in Regions 1 and 2.

 

“Strandings can be of natural causes, but when marine mammals with good body condition strand, we have to worry because it could imply that they are in trouble. It is possible that their habitat is compromised in various ways – most likely from human activities such as pollution and overexploitation of our key marine resources,” explained Dr. Aragones, who also serves as the president of the Philippine Marine Mammal Stranding Network (PMMSN).

 

While natural causes such as tropical monsoons and oceanographic factors can affect marine mammal strandings, the study emphasized that fisheries production and fisheries interaction are major contributors to the stranding frequencies of these mammals. This can occur through fishing equipment that increases the chances of entanglement or various illegal fishing activities, such as dynamite fishing.

 

“We recommend that LGUs and concerned regional offices establish their own stranding response team and rehabilitation tank and eradicate illegal fishing activities in their area by providing appropriate crew and patrol boats,” the researchers stated in their study. They also emphasized the importance of information, education, and communication (IEC) campaigns regarding marine mammals and regulating fishers and their fishing gear. By institutionalizing these programs, LGUs and concerned regional offices will help sustain their implementation.

Tala, a rough-toothed dolphin (Steno bredanensis), was rehabilitated by PMMSN for 51 days before being successfully released back into the Lingayen Gulf on August 21, 2018. (Photo credit: PMMSN)

Their study also revealed that the species most frequently stranded in Philippine waters were spinner dolphins (Stenella longirostris) with 218 strandings, dugongs (Dugong dugon) with 104 strandings, Risso’s dolphins (Grampus griseus) with 100 strandings, Fraser’s dolphins (Lagenodelphis hosei) with 95 strandings, and melon-headed whales (Peponocephala electra) with 89 strandings. By establishing a ‘species stranding status,’ an index of the impacts of stranding on these already vulnerable and endangered species, this study has introduced an important additional key factor in assessing species conservation status by concerned agencies.


Generating more research on stranded marine mammals


Knowing the species—particularly identifying their genetics and genomics—is important for ensuring the proper management of stranded marine mammals. This is the focus of the new ongoing studies by Dr. Aragones and his colleagues. 


“We should determine the main causes of strandings. Since we suspect that dynamite blasts can cause strandings, we want to understand the acoustics of marine mammals,” elaborated Dr. Aragones. “We also like to examine the impact of fisheries on our marine mammals, to identify what types of fishing gears can entangle them, and the bycatch rates as well.” 


Their findings underscore the importance of continuing and advancing monitoring efforts for marine mammal strandings, given the inevitable impacts of human activities such as increasing population, illegal fishing, pollution, and climate change. The evidence-based results provided by this study can guide stranded hotspot LGUs and concerned regional offices in managing and conserving Philippine marine resources, including marine mammals.


For interview requests and other concerns, please contact media@science.upd.edu.ph.


References:


Aragones, L. V., Morado, A. N., Obusan, M. C., Laggui, H. L., Bondoc, J. L., Suarez, L. J., & Lawler, E. K. (2024). Spatiotemporal variation of stranded marine mammals in the Philippines from 2005 to 2022: Latest stranding hotspots and species stranding status. Aquatic Mammals, 50(4), 302-322. https://doi.org/10.1578/am.50.4.2024.302

‘Death Is Not a Failure’: Enhancing Dolphin Disease Knowledge Through Hands-on Training

‘Death Is Not a Failure’: Enhancing Dolphin Disease Knowledge Through Hands-on Training

Published: July 05, 2024

By: Eunice Jean C. Patron

Veterinarians measuring the length of a spinner dolphin (Stenella coeruleoalba) (Photo credit: Eunice Jean Patron)

Albeit disheartening, scientists said the death of a stranded dolphin is not entirely a setback during the “Cetacean Pathology Training and Workshop: Pathogenesis of Common Diseases in Stranded Dolphins,” held on June 24, 2024, at the University of the Philippines – Diliman College of Science’s Institute of Environmental Science and Meteorology (UPD-CS IESM), Marine Mammal Research and Conservation Laboratory (MMRCL).


The workshop aimed to equip veterinarians with the proper practices and skills for documenting and collecting information about dolphin diseases, ensuring that a dolphin’s death contributes to deepening knowledge about these diseases. It was organized by the MMRCL of IESM, the Microbial Ecology of Terrestrial and Aquatic Systems Laboratory (METAS Lab) of the UPD-CS Institute of Biology (IB), the Philippine Marine Mammal Stranding Network (PMMSN), the Veterinary Practitioners Association of the Philippines (VPAP), and the Philippine Veterinary Medical Association (PVMA). 


“We conduct these sorts of training to build the capacity of local government units (LGUs) and concerned agencies in their marine mammal rehabilitation and post-mortem response,” said IESM professor and MMRCL head, Dr. Lemnuel Aragones, who is also the president of PMMSN.


Data presented in the workshop show that human activities, particularly underwater noise pollution such as blasts, significantly impact dolphins. Dr. Leo Jonathan Suarez, PMMSN treasurer and Head of Veterinary Services at Ocean Adventure, mentioned that apart from body tissue injury, blasts can also cause acoustic trauma. “The ear has the most sensitive tissues for pressure-induced damage,” he pointed out. “An adult may not experience or sustain injury from the blast itself. But, of course, the sound travels really far. So, they may actually suffer the consequences of the explosion through that sound.”


As Dr. Suarez showed several examples of suspected acoustic trauma cases, he reiterated that deaf dolphins can survive, yet they will slowly suffer due to hearing loss. “If they cannot echolocate, they cannot find and catch food. They cannot avoid predators. They cannot navigate. They cannot communicate. So basically, all of their skills and abilities to live in the wild are removed,” he added.


Focusing on studies of marine mammal diseases, PMMSN advisory member Dr. Christopher Torno, who mentioned that dolphin deaths are not truly failures, highlighted the importance of dolphin disease knowledge. “Sometimes when people talk about these things, take it with a grain of salt. Tignan niyo rin. As much as possible, I try to superimpose what I’ve learned. And you should too. ‘Yung mga templates natin sa pathology na you might think are useless, they are very useful,” he expounded.


Dr. Torno also reminded the workshop participants to double-check the dolphin’s organs when investigating the cause of its death, even if suspicions already exist. He shared how he has made mistakes during necropsies, illustrating that veterinarians can learn a lot about dolphin diseases through these examinations. ‘The information you’re bringing us will benefit not just you, but this entire network. We’re learning from this, and I’m so happy I can share it with you,’ he said.


Dr. Marie Christine Obusan, an IB professor and one of the heads of the METAS Lab, emphasized the need for more research on dolphin rehabilitation response. “Our country represents one-third of the world’s cetacean diversity,” she explained. “And there is an increasing trend of stranding events over the years.”


As a scientist mainly focused on assessing samples from marine mammal stranding events, Dr. Obusan shared her experiences working with veterinarians in the field. “I’m very appreciative of the inputs I’m getting from different experts and being mentored by different veterinarians,” she said, underscoring how collaborations with people from various fields and expertise, as well as citizens themselves, can be helpful in better understanding marine mammals such as dolphins.

Workshop organizers and participants gathered in front of the IESM building for a photo after the morning session lectures. (Photo credit: Eunice Jean Patron)

Veterinarians from different LGUs across the Philippines, the Bureau of Fisheries and Aquatic Resources (BFAR), state universities and colleges (SUCs), and private veterinary clinics participated in the workshop. After the morning session lectures by the speakers, the participating veterinarians conducted a dolphin necropsy in the MMRCL room during the afternoon session.


The workshop organizers have also collaborated on other medical management training programs for marine mammals. “The need never ends, as we know that the frequency of strandings continues to increase through the years,” Dr. Aragones mentioned. “We focus on topics we believe are lacking or needed to enhance local capacities.”


This workshop is part of the implementation of a research project on Cetacean health surveillance which is funded by the Department of Science and Technology – Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) through its Marine Resources Research Division.


Dr. Aragones and his team will soon release a scientific paper detailing marine mammal strandings in the Philippines from 2005 to 2022.

 

For interview requests and other concerns, please contact media@science.upd.edu.ph.

UP Mathematician Develops Model For Heat Propagation Through Rough Surfaces

UP Mathematician Develops Model For Heat Propagation Through Rough Surfaces

Published: July 02, 2024

By: Harvey L. Sapigao

Model for two materials with a rough interface (Photo Credit: Avila et. al. 2024)

Suppose you have a pair of pants you need to wear tomorrow. The problem is, you just washed them, and they’re still damp. So you resort to a handy tool: the flat iron.

 

Using the iron, you can easily dry the flat parts of the pants, but you will notice that it is more difficult to dry some areas where the surface is uneven – near the zipper, the side and back pockets, the seams, and the waistband.

 

That’s because the heat from the iron does not reach the uneven areas as quickly as it does the flatter areas. In other words, the heat propagation, or the heat transfer from the iron to the pants, is not uniform on rough surfaces.

 

Understanding how heat propagates from one material to another is important in designing efficient cooling devices, such as air conditioners, or constructing infrastructures that minimize heat from the sun. In some cases, heat propagation is simple to model when the boundary at which the two materials touch is flat, such as ironing the flat areas of the pants. In other cases, however, the heat propagation model is more complex when the boundary is rough, such as when ironing the uneven areas of the pants.

 

Jake Avila of the UPD College of Science Institute of Mathematics (UPD-CS IM) formulated a theory to model heat propagation through rough surfaces. With the help of mathematicians in Italy, Avila’s theory uses a method called homogenization.

 

Homogenization is a way of combining the tiny details of the material with its bigger, more uniform properties. For example, the flat areas of the pants may look flat under the naked eye, but if you examine them under a microscope, you will notice the individual bumps made by the interwoven threads, which don’t look flat at all. “Homogenization theory aims to describe the macroscopic or effective properties of composite materials while taking into account simultaneously the microscopic or local properties of its components,” explained Avila.

 

Homogenization helps simplify the problem and provides a more accurate model for heat propagation. According to  Avila, homogenization incorporates the effective properties of the material, such as the thermal conductivity, while also considering the individual features of the material’s components.

 

Although he only applied his theory to heat propagation, it can be used for other physical phenomena as well. “[It] can also be applied to describe the acoustic wave propagation over rough walls or the turbulence flow in the rough ocean surface,” Avila said.

 

The next step is to develop a more general version of the theory for wider application. “The next plan is to study a more complicated and general problem wherein a sign-changing density function is involved in the eigenvalue problem in a domain through rough surfaces,” said  Avila. “The problem in the article is a special case where a unit density function is treated.”

For interview requests and other concerns, please contact media@science.upd.edu.ph.


References: Avila, J., Monsurrò, S., & Raimondi, F. (March 2023). Homogenization of an eigenvalue problem through rough surfaces. Asymptotic Analysis, 1–25. https://doi.org/10.3233/asy-231882